Optimizing CRISPR/Cas9 for the Diatom Phaeodactylum tricornutum

Optimizing CRISPR/Cas9 for the Diatom Phaeodactylum tricornutum

Blog Article

CRISPR/Cas9 is a powerful tool for read more genome editing.We constructed an easy-to-handle expression vector for application in the model organism Phaeodactylum tricornutum and tested its capabilities in order to apply CRISPR/Cas9 technology for our purpose.In our experiments, we targeted two different genes, screened for mutations and analyzed mutated diatoms in a three-step process.

In the end, we identified cells, showing either monoallelic or homo-biallelic targeted mutations.Thus, we confirm that application of the CRISPR/Cas9 system tonic shower cap for P.tricornutum is very promising, although, as discussed, overlooked pitfalls have to be considered.